Journal: European Radiology Experimental

Article Title: [Fe-EOB-tCDTA] generates strong contrast in the blood but not in the liver, despite inhibiting cellular [Gd-EOB-DTPA] 2- uptake and partial liver excretion

doi: 10.1186/s41747-026-00731-0

Figure Lengend Snippet: Toxicity of [Fe-EOB-tCDTA] and [Gd-EOB-DTPA] 2 - in BRL-3A cells. The cells were exposed to different concentrations of contrast agents (0, 1, 2.5, 5, 10, and 20 mM) for 2 h. Viability was determined using the MTT assay after incubation (three replicates, n = 9, mean ± standard error). Statistical analyses were performed using multiple unpaired t -tests. No significant differences were observed between the various concentration groups

Article Snippet: BRL-3A cells (ATCC, Manassas, VA, USA) were grown under the following conditions: DMEM/F12 medium containing GlutaMAX, 10% fetal bovine serum, 1,000 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific) at 37 °C with 5% CO2.

Techniques: MTT Assay, Incubation, Concentration Assay

Journal: European Radiology Experimental

Article Title: [Fe-EOB-tCDTA] generates strong contrast in the blood but not in the liver, despite inhibiting cellular [Gd-EOB-DTPA] 2- uptake and partial liver excretion

doi: 10.1186/s41747-026-00731-0

Figure Lengend Snippet: Laser ablation–inductively coupled plasma–mass spectrometry of [Fe-EOB- t CDTA] and [Gd-EOB-DTPA] 2 - in BRL-3A cells. a 56 Fe mass spectrometry signal of endogenous iron and of [Fe-EOB- t CDTA]; b 158 Gd mass spectrometry signal of [Gd-EOB-DTPA] -2 . The BRL-3A cells were treated with [Gd-EOB-DTPA] 2 - only (1 mM) or with [Gd-EOB-DTPA] 2 - and [Fe-EOB- t CDTA] at different molar ratios (1:0.5, 1:1, 1:2.5, 1:5, 1:10, and 1:20). Blank: No metal complexes.

Article Snippet: BRL-3A cells (ATCC, Manassas, VA, USA) were grown under the following conditions: DMEM/F12 medium containing GlutaMAX, 10% fetal bovine serum, 1,000 U/mL penicillin, and 100 μg/mL streptomycin (Gibco, Thermo Fisher Scientific) at 37 °C with 5% CO2.

Techniques: Clinical Proteomics, Mass Spectrometry

Journal: Comprehensive Physiology

Article Title: Cardiovascular β‐Adrenergic Receptor Distribution and Function: Influence of Species, Sex, Age, and Tissue

doi: 10.1002/cph4.70159

Figure Lengend Snippet: Vasorelaxant effects of β‐AR agonists in thoracic aorta from SHRs and wild‐type C57BL6 mice. The graphs illustrate the vasorelaxant responses of female and male aortic rings, with and without endothelium, to β‐AR agonists. Panel (A) shows the effects of the β 1 /β 2 ‐AR agonist isoprenaline and the β 3 ‐AR agonist BRL‐37344 in female and male SHRs. Significant sex differences are observed in SHRs, and these differences are endothelium‐dependent, as removal of the endothelium abolishes the enhanced responses in females. Panel (B) depicts the effects of the same β‐AR agonists in female and male wild‐type mice, where no significant sex differences are detected, and endothelial removal has no measurable effect. Experiments were performed with n = 6 for both SHRs and wild‐type mice. All values are presented as mean ± SD. Panel (A) is created based on data from Al‐Gburi et al. .

Article Snippet: Following pre‐constriction with phenylephrine (PE; 10 −6 M; Sigma‐Aldrich, P6126), cumulative concentration‐response curves were generated using the β1/β2‐AR agonist isoprenaline (10 −11 –10 −6 M; Tocris Bioscience, 1747) or the β3‐AR agonist BRL 37344 sodium salt (10 −9 –10 −4 M; Tocris Bioscience, 0948) according to Al‐Gburi et al. ( ).

Techniques: